7RQ9

Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	208.800, 444.650, 619.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.530 - 2.600
R-factor	0.2438
Rwork	0.241
R-free	0.29780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhv
RMSD bond length	0.005
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	106.530	106.530	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.188	0.062	1.401
Rmeas	0.211	0.069	1.595
Total number of observations	7853375
Number of reflections	1706765	19537	123882
<I/σ(I)>	6.76	19.3	0.96
Completeness [%]	98.0	95	96.5
Redundancy	4.601	5.341	4.272
CC(1/2)	0.989	0.996	0.125

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME