7RPY
X25-2 domain of Sca5 from Ruminococcus bromii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-05 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 100.820, 100.820, 87.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.460 - 1.670 |
R-factor | 0.1823 |
Rwork | 0.182 |
R-free | 0.19630 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.350 |
Data reduction software | HKL-2000 |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.460 | 1.730 |
High resolution limit [Å] | 1.670 | 1.670 |
Number of reflections | 60154 | 5957 |
<I/σ(I)> | 17.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.2 | 9 |
CC(1/2) | 1.000 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2M Ammonium sulfate, 0.1M NaAcetate pH=4.6 |