7RPP
Crystal structure of human CEACAM1 with GFCC' and ABED face
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.130, 86.060, 66.080 |
| Unit cell angles | 90.00, 100.27, 90.00 |
Refinement procedure
| Resolution | 29.860 - 2.200 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qxw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.773 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.860 | 29.860 | 2.320 |
| High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
| Rmerge | 0.133 | 0.046 | 0.767 |
| Rmeas | 0.162 | 0.054 | 0.952 |
| Rpim | 0.071 | 0.023 | 0.431 |
| Total number of observations | 101329 | 3417 | 13993 |
| Number of reflections | 20512 | 668 | 2973 |
| <I/σ(I)> | 7.3 | 16.7 | 2.1 |
| Completeness [%] | 100.0 | 98.9 | 100 |
| Redundancy | 4.9 | 5.1 | 4.7 |
| CC(1/2) | 0.992 | 0.996 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
