7RP0

Structural Snapshots of Intermediates in the Gating of a K+ Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000032
Spacegroup name	I 4
Unit cell lengths	155.330, 155.330, 76.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.917 - 2.480
R-factor	0.2444
Rwork	0.241
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.980	2.580
High resolution limit [Å]	2.478	2.480
Number of reflections	58647	3643
<I/σ(I)>	13.2
Completeness [%]	99.9
Redundancy	7.4
CC(1/2)	0.990	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25	293	50 mM MES, pH 6.25, 50 mM magnesium acetate, 27% PEG400