7RMM

Structure of N74D mutant of disulfide stabilized HIV-1 CA hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-10-13
Detector	RDI CMOS_8M
Wavelength(s)	1.0000
Spacegroup name	P 6
Unit cell lengths	157.360, 157.360, 56.575
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.430 - 1.970
R-factor	0.2192
Rwork	0.218
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vkv
RMSD bond length	0.006
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.430	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.106	1.308
Number of reflections	56827	4002
<I/σ(I)>	15.8	1.6
Completeness [%]	100.0	100
Redundancy	10.9	10.2
CC(1/2)	0.999	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2M NaI, 5% peg 3350, 6% glycerol, 0.1M sodium cacodylate trihydrate pH 6.5