7RM1

Antibody 2F2 in complex with P. vivax CSP peptide EDGAGNQPGANGAGNQPGANGAGNQPG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033167
Spacegroup name	P 31
Unit cell lengths	142.420, 142.420, 91.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.540 - 3.190
R-factor	0.1813
Rwork	0.180
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uyw
RMSD bond length	0.011
RMSD bond angle	1.394
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.550	3.300
High resolution limit [Å]	3.190	3.190
Number of reflections	34411	3469
<I/σ(I)>	13.2	1.7
Completeness [%]	99.8	99.6
Redundancy	20.1	20.3
CC(1/2)	0.998	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.08 M sodium acetate pH 4.6, 20% glycerol (v/v) and 1.6 M ammonium sulfate