7RLY
Antibody 2F2 in complex with P. vivax CSP peptide DRAAGQPAGDRADGQPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033167 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.723, 82.287, 82.747 |
| Unit cell angles | 95.26, 113.78, 111.47 |
Refinement procedure
| Resolution | 29.610 - 2.670 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xiw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.610 | 2.770 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Number of reflections | 43179 | 4542 |
| <I/σ(I)> | 4.7 | 1.5 |
| Completeness [%] | 98.3 | 97.8 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.987 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M sodium citrate pH 5.0 and PEG 6000 |
