7RLX

Antibody 2F2 in complex with P. vivax CSP peptide GDRADGQPAGDRAAGQPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-02-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033167
Spacegroup name	C 1 2 1
Unit cell lengths	92.615, 60.810, 81.352
Unit cell angles	90.00, 101.57, 90.00

Refinement procedure

Resolution	29.710 - 1.970
R-factor	0.1884
Rwork	0.186
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xiw
RMSD bond length	0.015
RMSD bond angle	1.324
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.710	2.020
High resolution limit [Å]	1.970	1.970
Number of reflections	31038	1962
<I/σ(I)>	8.3	1.5
Completeness [%]	98.7
Redundancy	3.3
CC(1/2)	0.996	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M potassium acetate and 20% (w/v) PEG 3350