7RLV

Antibody 2F2 in complex with P. vivax CSP peptide GDRADGQPAGDRADGQPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033167
Spacegroup name	P 1
Unit cell lengths	71.487, 81.425, 82.263
Unit cell angles	94.59, 114.10, 111.60

Refinement procedure

Resolution	29.480 - 2.200
R-factor	0.1814
Rwork	0.179
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xiw
RMSD bond length	0.007
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.480	2.250
High resolution limit [Å]	2.200	2.200
Number of reflections	75088	4440
<I/σ(I)>	5.2	1.5
Completeness [%]	97.5	97.1
Redundancy	3.5	3.1
CC(1/2)	0.965	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M di-sodium tartrate and 20% (w/v) PEG 3350