7RLM
Crystal Structure of Potato Leafroll Virus (PLRV) Coat Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-12 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.863, 67.479, 75.887 |
| Unit cell angles | 79.67, 76.22, 81.67 |
Refinement procedure
| Resolution | 72.860 - 1.800 |
| R-factor | 0.1698 |
| Rwork | 0.167 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sco |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.860 | 1.558 |
| High resolution limit [Å] | 1.504 | 1.504 |
| Rmerge | 0.072 | |
| Number of reflections | 177750 | 6323 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 89.1 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.997 | 0.315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Bis-Tris propane, pH 7.7, 26% PEG3350, 0.1 M sodium bromide |
