7RJQ

Crystal structure of human Bromodomain containing protein 4 (BRD4) in complex with ILF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	43.741, 57.115, 123.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.440 - 1.720
R-factor	0.1809
Rwork	0.179
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mxf
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.750
High resolution limit [Å]	1.720	4.660	1.720
Rmerge	0.100	0.028	0.924
Rmeas	0.108	0.030	1.004
Rpim	0.041	0.012	0.388
Total number of observations	110235
Number of reflections	16699	918	790
<I/σ(I)>	8.4
Completeness [%]	98.9	97.9	99
Redundancy	6.6	6.5	6.6
CC(1/2)		0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	3.0 M sodium chloride, 0.1 M HEPES