7RJM

Crystal structure of human Bromodomain containing protein 3 (BRD3) in complex with ILF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	59.009, 59.009, 133.966
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.870 - 2.100
R-factor	0.194
Rwork	0.192
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qju
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.103	24.617	2.210
High resolution limit [Å]	2.100	6.640	2.100
Rmerge		0.039	0.575
Rmeas	0.064	0.041	0.607
Rpim	0.021	0.015	0.192
Total number of observations	155473	4714	23218
Number of reflections	16445	589	2343
<I/σ(I)>	19.5	42.4	4.1
Completeness [%]	99.9	98	100
Redundancy	9.5	8	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	25% w/v PEG3350, 0.1 M Bis-Tris, 0.2 M sodium chloride