7RJL

Crystal structure of human Bromodomain containing protein 3 (BRD3) in complex with SHMT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	51.550, 61.572, 83.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.790 - 1.500
R-factor	0.1584
Rwork	0.157
R-free	0.18470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qju
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (dev_3940)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.890	28.890	1.530
High resolution limit [Å]	1.500	8.210	1.500
Rmerge	0.086	0.058	0.702
Rmeas	0.096	0.066	0.783
Rpim	0.041	0.030	0.338
Total number of observations	239500	1498	9413
Number of reflections	43125	314	1873
<I/σ(I)>	10.9	23	2.3
Completeness [%]	99.3	97.5	87.8
Redundancy	5.6	4.8	5
CC(1/2)	0.996	0.993	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	35% v/v Tacsimate