7RJ3

Crystal Structure of the Forkhead Associated (FHA) Domain of the Glycogen Accumulation Regulator (GarA) from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	50.135, 56.755, 92.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.130 - 1.680
R-factor	0.1769
Rwork	0.174
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i2p
RMSD bond length	0.006
RMSD bond angle	1.395
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.065	0.789
Rmeas	0.070	0.854
Rpim	0.027	0.325
Number of reflections	30373	1511
<I/σ(I)>	26.9	2.61
Completeness [%]	100.0	100
Redundancy	6.8	6.8
CC(1/2)		0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	292	15.3 mg/mL protein in 0.5 M sodium chloride, 0.01 M Tris, pH 8.3 against JCSG+ screen G10 (0.15 M potassium bromide, 30% w/v PEG2000 MME)