7RIP

RNA polymerase II elongation complex with hairpin polyamide Py-Im 1, scaffold 1 soaked with CTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-08-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	168.158, 223.041, 193.464
Unit cell angles	90.00, 100.67, 90.00

Refinement procedure

Resolution	49.225 - 3.300
R-factor	0.2198
Rwork	0.219
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rim
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.230	49.230	3.330
High resolution limit [Å]	3.270	17.910	3.270
Rmerge	0.380	0.031	4.013
Rmeas	0.408	0.034	4.302
Rpim	0.147	0.012	1.546
Total number of observations	829562	4799	41475
Number of reflections	107923	658	5363
<I/σ(I)>	6.7	51.9	0.6
Completeness [%]	100.0	95.3	100
Redundancy	7.7	7.3	7.7
CC(1/2)	0.988	0.999	0.239

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000