7RIM

RNA polymerase II elongation complex with hairpin polyamide Py-Im 1, scaffold 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9999
Spacegroup name	C 1 2 1
Unit cell lengths	167.220, 221.930, 193.710
Unit cell angles	90.00, 99.63, 90.00

Refinement procedure

Resolution	82.432 - 2.900
R-factor	0.217
Rwork	0.215
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uq2
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall
Low resolution limit [Å]	82.432
High resolution limit [Å]	2.900
Number of reflections	153630
<I/σ(I)>	5.83
Completeness [%]	99.7
Redundancy	2
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000