7RHT

Importin alpha 7 delta IBB (KPNA6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	64.850, 75.840, 88.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.770 - 2.500
R-factor	0.2105
Rwork	0.209
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uad
RMSD bond length	0.003
RMSD bond angle	0.491
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.770	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.134	0.958
Number of reflections	15096	1703
<I/σ(I)>	8.1	2
Completeness [%]	97.2
Redundancy	5.1
CC(1/2)	0.992	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES monohydrate pH 6.5, 12% PEG20000