7RHM
Structure of Q67H/N74D mutant of disulfide stabilized HIV-1 CA hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-10-16 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 6 |
Unit cell lengths | 92.116, 92.116, 57.837 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.830 - 2.160 |
R-factor | 0.2327 |
Rwork | 0.231 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h47 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.418 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.830 | 2.230 |
High resolution limit [Å] | 2.160 | 2.160 |
Number of reflections | 15160 | 1303 |
<I/σ(I)> | 10.8 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 10.8 |
CC(1/2) | 0.995 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.35M NaI, 3% peg 3350, 6% glycerol, 0.1M sodium cacodylate trihydrate pH 6.5 |