7RGA
Crystal structure of nanoCLAMP3:VHH in complex with MTX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-10-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95373 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 173.200, 143.969, 181.814 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 48.380 - 2.900 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.24223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QXV 2W1Q |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.057 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.380 | 2.975 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 1401844 | 70152 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 14.5 |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.4 M sodium malonate-malonic acid pH 7.0 |
