7RG5

Importin alpha3 in complex with p50 NLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-09-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	47.751, 59.183, 86.239
Unit cell angles	90.00, 96.32, 90.00

Refinement procedure

Resolution	48.700 - 2.150
R-factor	0.2275
Rwork	0.226
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvz
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.720	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.054	0.410
Rpim	0.039	0.310
Number of reflections	26271	2043
<I/σ(I)>	13.4	2.7
Completeness [%]	99.6
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.2M potassium thiocyanate and 20% PEG3350