7RG5
Importin alpha3 in complex with p50 NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-09-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.751, 59.183, 86.239 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 48.700 - 2.150 |
| R-factor | 0.2275 |
| Rwork | 0.226 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bvz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.482 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.720 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.054 | 0.410 |
| Rpim | 0.039 | 0.310 |
| Number of reflections | 26271 | 2043 |
| <I/σ(I)> | 13.4 | 2.7 |
| Completeness [%] | 99.6 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.2M potassium thiocyanate and 20% PEG3350 |
