7RFY

Importin alpha3 in complex with MERS ORF4B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-08-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	48.222, 59.711, 82.640
Unit cell angles	90.00, 98.89, 90.00

Refinement procedure

Resolution	25.990 - 2.500
R-factor	0.2497
Rwork	0.249
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvz
RMSD bond length	0.003
RMSD bond angle	0.595
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	autoBUSTER (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.990	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.110	0.870
Number of reflections	15900	1762
<I/σ(I)>	5.4	1.2
Completeness [%]	98.2
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.2 M magnesium chloride, 0.1 M Tris pH 8.0, 20% w/v PEG 6000