7RFQ

STRUCTURE OF BACTERIAL SYLF DOMAIN CONTAINING PROTEIN, BETA CELL EXPANSION FACTOR A (BEFA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-07-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0, 0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	94.670, 64.550, 42.630
Unit cell angles	90.00, 113.26, 90.00

Refinement procedure

Resolution	37.350 - 1.270
R-factor	0.132
Rwork	0.131
R-free	0.15500
Structure solution method	SIRAS
Data reduction software	HKL-2000
Data scaling software	SCALA (3.3.22)
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.350	1.320
High resolution limit [Å]	1.270	1.270
Rmerge		0.114
Rmeas	0.177	0.119
Rpim	0.054	0.035
Total number of observations	621902	23389
Number of reflections	62064	2032
<I/σ(I)>	8.7	17.2
Completeness [%]	100.0	99.9
Redundancy	10	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	295	25 % PEG 3350, 100 mM CITRIC ACID PH 3.5, 1 mM TCEP, 39 mM O-PHOSPHO-L-SERINE OR 39 mM SERINE, VAPOR