7RF0

MYCOBACTERIUM ABSCESSUS TRNA METHYLTRANSFERASE IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND MAGNESIUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9802
Spacegroup name	P 21 21 21
Unit cell lengths	74.398, 78.835, 86.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.220 - 1.590
R-factor	0.1819
Rwork	0.180
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nvr
RMSD bond length	0.006
RMSD bond angle	0.980
Data reduction software	ADDREF
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.240	1.600
High resolution limit [Å]	1.560	1.560
Number of reflections	68820	4995
<I/σ(I)>	18.8	1.9
Completeness [%]	99.2
Redundancy	6.6
CC(1/2)	0.999	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Drops were 2ul of protein 24 mg/ml and 2ul buffer (0.1M sodium cacodylate and 2M ammonium nitrate pH 6.5); co-crystallized with SAH in a 1:1 ratio at a final concentration of 5mM SAH in the drops; and 4ul MgSO4 at 10mM were added to the drops before cryo-cooling