7RDS
Structure of human NTHL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-01 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 63 |
| Unit cell lengths | 124.800, 124.800, 42.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.850 - 2.500 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.24450 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.431 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.850 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.077 | 0.619 |
| Number of reflections | 13457 | 1567 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.2 | 22.3 |
| CC(1/2) | 0.993 | 0.181 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 5K MME, Sodium chloride, Tricine |
