7RD5

Crystal structure of Tspan15 large extracellular loop (Tspan15 LEL) in complex with 1C12 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	171.300, 124.640, 116.120
Unit cell angles	90.00, 98.17, 90.00

Refinement procedure

Resolution	47.500 - 3.600
R-factor	0.2735
Rwork	0.271
R-free	0.30930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bdz
RMSD bond length	0.003
RMSD bond angle	0.736
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	3.729
High resolution limit [Å]	3.600	3.600
Rmerge	0.158	1.080
Rmeas	0.199	1.364
Rpim	0.120	0.822
Number of reflections	27150	6908
<I/σ(I)>	4.63	0.86
Completeness [%]	95.8	97.17
Redundancy	2.5	2.5
CC(1/2)	0.995	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M sodium acetate pH 4.6, 3.4 M sodium nitrate