7RCU

Synthetic Max homodimer mimic in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-10-20
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	25.666, 46.266, 166.017
Unit cell angles	90.05, 90.05, 90.02

Refinement procedure

Resolution	46.270 - 2.690
R-factor	0.2395
Rwork	0.235
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hlo
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.690	7.320	2.700
Rmerge	0.030	0.018	0.514
Rmeas	0.042	0.026	0.727
Rpim	0.030	0.018	0.514
Number of reflections	18936	943	906
<I/σ(I)>	13.3
Completeness [%]	89.4	87.6	81
Redundancy	1.8	1.9	1.8
CC(1/2)		0.997	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	50 mM Tris pH 7.0, 30% 2-Methyl-2,4-pentanediol, 50 mM NaCl and 10 mM MgCl2