7RCS

Crystal structure of PfCSP peptide 21 with vaccine-elicited human anti-malaria antibody m43.160

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	54.768, 57.518, 74.873
Unit cell angles	85.80, 77.43, 72.64

Refinement procedure

Resolution	44.400 - 2.400
R-factor	0.1914
Rwork	0.188
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b5l
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.055	0.023	0.200
Rmeas	0.074	0.031	0.279
Rpim	0.049	0.020	0.195
Total number of observations	55917
Number of reflections	27527	1424	1013
<I/σ(I)>	10
Completeness [%]	82.7	85.4	61.5
Redundancy	2	2	1.7
CC(1/2)		0.997	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis-Tris, pH 5.5, 25% w/v PEG3350