7RCO
Crystal structure of human TGF-beta-2 bound to 4A11.V2 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 60.154, 103.479, 196.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.951 - 2.900 |
| R-factor | 0.2119 |
| Rwork | 0.208 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tfg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.229 |
| Data reduction software | AutoProcess |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12-2829_final) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.060 | 49.060 | 3.080 |
| High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
| Rmerge | 0.086 | 0.033 | 0.731 |
| Rmeas | 0.099 | 0.039 | 0.831 |
| Rpim | 0.048 | 0.021 | 0.389 |
| Total number of observations | 111744 | 3561 | 18905 |
| Number of reflections | 27630 | 1074 | 4429 |
| <I/σ(I)> | 11.6 | 29.5 | 1.9 |
| Completeness [%] | 98.8 | 93.1 | 99.7 |
| Redundancy | 4 | 3.3 | 4.3 |
| CC(1/2) | 0.998 | 0.998 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG4000, 0.1 M sodium cacodylate, pH 6.0 |
