7RCL

Crystal Structure of ADP-bound Galactokinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-21
Detector	ADSC QUANTUM 1
Wavelength(s)	1.1533
Spacegroup name	P 21 21 21
Unit cell lengths	96.901, 114.126, 206.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.630 - 2.400
R-factor	0.1743
Rwork	0.173
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wuu
RMSD bond length	0.013
RMSD bond angle	2.007
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.225	0.107
Rmeas	0.222	0.110
Rpim	0.069	0.025	0.797
Total number of observations	955737
Number of reflections	86404	9459	8520
<I/σ(I)>	9
Completeness [%]	95.7	100	95
Redundancy	11.1	19.2	6.1
CC(1/2)		0.998	0.356

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	286	Seeding into drops containing GALK protein at 8 mg/ml, 2.3M Na/K pH6.8