7RCL
Crystal Structure of ADP-bound Galactokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.1533 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.901, 114.126, 206.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.630 - 2.400 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wuu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.007 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.225 | 0.107 | |
| Rmeas | 0.222 | 0.110 | |
| Rpim | 0.069 | 0.025 | 0.797 |
| Total number of observations | 955737 | ||
| Number of reflections | 86404 | 9459 | 8520 |
| <I/σ(I)> | 9 | ||
| Completeness [%] | 95.7 | 100 | 95 |
| Redundancy | 11.1 | 19.2 | 6.1 |
| CC(1/2) | 0.998 | 0.356 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 286 | Seeding into drops containing GALK protein at 8 mg/ml, 2.3M Na/K pH6.8 |
