7RBW

Structure of Biliverdin-binding Serpin of Boana punctata (polka-dot tree frog)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	72.069, 75.910, 143.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 2.050
R-factor	0.201
Rwork	0.199
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nbu
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.970	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.084	0.725
Number of reflections	50138
<I/σ(I)>	18.2
Completeness [%]	99.8	99.9
Redundancy	12.8	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	292	0.2 M Ammonium nitrate, 20% w/v Polyethylene glycol 3,350