7RBL
Human DNA polymerase beta crosslinked complex, 60 s Ca to Mg exchange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.306, 81.394, 55.534 |
| Unit cell angles | 90.00, 108.01, 90.00 |
Refinement procedure
| Resolution | 40.730 - 1.980 |
| R-factor | 0.1902 |
| Rwork | 0.188 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rpy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.512 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.840 | 47.840 | 2.030 |
| High resolution limit [Å] | 1.980 | 9.070 | 1.980 |
| Rmerge | 0.066 | 0.043 | 0.524 |
| Rmeas | 0.072 | 0.046 | 0.574 |
| Rpim | 0.027 | 0.017 | 0.230 |
| Total number of observations | 198825 | 2165 | 11756 |
| Number of reflections | 29288 | 322 | 1985 |
| <I/σ(I)> | 15.1 | 35.8 | 3.2 |
| Completeness [%] | 98.6 | 97.7 | 95.7 |
| Redundancy | 6.8 | 6.7 | 5.9 |
| CC(1/2) | 0.999 | 0.998 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
