7R9D
Crystal structure of Nb_0 in complex with Fab_8D3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.510, 60.287, 77.244 |
| Unit cell angles | 90.00, 109.34, 90.00 |
Refinement procedure
| Resolution | 28.450 - 1.830 |
| R-factor | 0.1808 |
| Rwork | 0.180 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Fab with similar sequence |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v.1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.068 | 0.550 |
| Number of reflections | 49251 | 3189 |
| <I/σ(I)> | 16.46 | |
| Completeness [%] | 99.3 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M Ammonium formate, 20-22 % w/v PEG 3350 |
