7R8U

LOX-1 - Structural and Functional Studies of a Receptor Implicated in Atherosclerosis

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-01-28
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.19499
Spacegroup name	P 21 21 21
Unit cell lengths	71.908, 89.901, 110.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.492 - 1.901
Rwork	0.186
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ypu
RMSD bond length	0.009
RMSD bond angle	1.611
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.492	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	57364	3538
<I/σ(I)>	1.6
Completeness [%]	96.9
Redundancy	6.5
CC(1/2)	0.995	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M 1,2-propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol, 1 M Tris base, BICINE, pH 8.5, 50% v/v precipitant mix (25% v/v MPD, 25% PEG1000, 25% w/v PEG3350)