7R7O

Structure of methyltransferase domain of Spb1 boudn to SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	140.714, 45.497, 92.031
Unit cell angles	90.00, 123.42, 90.00

Refinement procedure

Resolution	42.420 - 1.580
R-factor	0.2297
Rwork	0.229
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6elz
RMSD bond length	0.007
RMSD bond angle	1.058
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.580	1.580
Rmeas	0.109
Rpim	0.037	0.037
Number of reflections	66501	3158
<I/σ(I)>	18.1	0.93
Completeness [%]	83.1
Redundancy	8.1
CC(1/2)	0.982	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20 mM Hepes KOH pH 8 150 mM KCl 0.5 mM TCEP 5% Glycerol 15% PEG 3350