7R7O
Structure of methyltransferase domain of Spb1 boudn to SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.714, 45.497, 92.031 |
| Unit cell angles | 90.00, 123.42, 90.00 |
Refinement procedure
| Resolution | 42.420 - 1.580 |
| R-factor | 0.2297 |
| Rwork | 0.229 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6elz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmeas | 0.109 | |
| Rpim | 0.037 | 0.037 |
| Number of reflections | 66501 | 3158 |
| <I/σ(I)> | 18.1 | 0.93 |
| Completeness [%] | 83.1 | |
| Redundancy | 8.1 | |
| CC(1/2) | 0.982 | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20 mM Hepes KOH pH 8 150 mM KCl 0.5 mM TCEP 5% Glycerol 15% PEG 3350 |
