7R79

Histoplasma capsulatum H88 Calcium Binding Protein 1 (Cbp1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11
Spacegroup name	C 1 2 1
Unit cell lengths	88.550, 46.697, 71.255
Unit cell angles	90.00, 109.10, 90.00

Refinement procedure

Resolution	67.330 - 1.600
R-factor	0.2179
Rwork	0.217
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Paracocci Cbp1
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.330	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.042	0.917
Rmeas	0.046
Rpim	0.018
Number of reflections	36276	24131
<I/σ(I)>	21.74
Completeness [%]	99.3
Redundancy	6.6
CC(1/2)	1.000	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.0 M Lithium chloride, 0.1 M Citric Acid, 20% PEG 6000