7R65
Crystal structure of a bacterial cyclic UMP synthase from Burkholderia cepacia LK29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-11-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.145, 66.758, 92.350 |
| Unit cell angles | 90.00, 111.26, 90.00 |
Refinement procedure
| Resolution | 45.220 - 1.450 |
| R-factor | 0.1774 |
| Rwork | 0.177 |
| R-free | 0.19760 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.638 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.960 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rpim | 0.029 | 0.872 |
| Number of reflections | 167231 | 7966 |
| <I/σ(I)> | 16.8 | 1.6 |
| Completeness [%] | 99.7 | 96.8 |
| Redundancy | 48.9 | 40.6 |
| CC(1/2) | 0.999 | 0.181 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M ammonium acetate, 0.1 M HEPES-KOH pH 7.5, and 29% PEG-3350 |
