7R63
Nb82, a nanobody against voltage gated sodium channels Nav1.4 and Nav1.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.745, 82.474, 170.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.650 - 2.000 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h6j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.577 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.670 | 29.650 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.072 | 0.034 | 0.394 |
| Rmeas | 0.078 | 0.037 | 0.428 |
| Rpim | 0.030 | 0.015 | 0.164 |
| Total number of observations | 2845 | 18213 | |
| Number of reflections | 37476 | 473 | 2739 |
| <I/σ(I)> | 14.5 | 31.2 | 4.2 |
| Completeness [%] | 99.9 | 97.6 | 100 |
| Redundancy | 6.7 | 6 | 6.6 |
| CC(1/2) | 0.999 | 0.999 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 295 | 1-5% PEG MME 550, 0.1M Bis Tris, 2.2M Ammonium sulphate |
