7R5N
Crystal structure of the full-length short LOV protein PF5-LOV from Pseudomonas fluorescens (dark state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 148.698, 148.698, 200.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.120 - 3.450 |
| R-factor | 0.2168 |
| Rwork | 0.215 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3w |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 3.780 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.081 | |
| Rmeas | 0.091 | |
| Rpim | 0.041 | |
| Number of reflections | 15076 | 3526 |
| <I/σ(I)> | 9 | 1 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 4.8 | 4.9 |
| CC(1/2) | 0.997 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287.15 | 1.2 M (NH4)2SO4, 0.1M MES |
