7R49

Crystal structure of the L. plantarum acyl carrier protein synthase (AcpS)in complex with D-alanyl carrier protein (DltC1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	68.537, 94.888, 98.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.790 - 1.880
R-factor	0.2176
Rwork	0.216
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f80
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.790	1.920
High resolution limit [Å]	1.880	1.880
Rpim	0.063	0.569
Number of reflections	52945	3096
<I/σ(I)>	9.1	1.4
Completeness [%]	99.3
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3350, Sodium Malonate