7R3P
Apoform of the periplasmic FtrA/P19 protein from Rubrivivax gelatinosus (His-tag)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.160, 80.300, 82.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.120 - 1.400 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lzl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.086 | |
| Number of reflections | 78363 | 12299 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 13 | |
| CC(1/2) | 0.999 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 2 M AS, 0.1 M citrate sodium |
