7R3B
S-adenosylmethionine synthetase from Lactobacillus plantarum complexed with AMPPNP, methionine and SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-03 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.426, 110.927, 112.661 |
| Unit cell angles | 93.82, 104.03, 99.59 |
Refinement procedure
| Resolution | 29.410 - 2.820 |
| R-factor | 0.16143 |
| Rwork | 0.156 |
| R-free | 0.27492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p7l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.546 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 2.921 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmeas | 0.114 | 0.700 |
| Number of reflections | 61578 | 4103 |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 94.6 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.996 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50mM CaCl2 0.1M Bis-Tris pH 6.8 32.14% PEG MME 550 |
