7R2M

SYNJ2BP complex with a synthetic Vangl2 peptide (9mer).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	60.270, 60.710, 143.150
Unit cell angles	90.00, 92.88, 90.00

Refinement procedure

Resolution	47.656 - 2.400
R-factor	0.2163
Rwork	0.215
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jik
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.656	47.656	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.130	0.036	1.291
Rmeas	0.140	0.039	1.387
Total number of observations	286773
Number of reflections	39408	489	2928
<I/σ(I)>	10.15	37.05	1.22
Completeness [%]	96.5	97.2	97.9
Redundancy	7.277	6.558	7.448
CC(1/2)	0.997	0.999	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Ammonium sulfate, 0.1 M Sodium formate, 0.1 M HEPES 7.0, 25 % v/v PEG Smear Broad