7R0O
Structure of a cytosolic sulfotransferase of Anopheles gambiae (AGAP001425).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2018-09-29 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.773, 92.147, 101.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.780 - 2.000 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fmj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.495 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.780 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.394 |
| Number of reflections | 50299 | 9858 |
| <I/σ(I)> | 8.4 | 1.95 |
| Completeness [%] | 99.0 | 99.28 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350, 0.3 M NaCl, Bis-tris 0.1 M pH 5.5 |
