7R0N
KRasG12C in complex with GDP and compound 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.351, 59.228, 65.207 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.500 - 1.200 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WXEX |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.210 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.039 | 0.696 |
| Number of reflections | 50779 | 1016 |
| <I/σ(I)> | 19.8 | 2.24 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.22 | 5.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 33% PEG 6000, 0.01 M NaCitrate |
