7R0K
Crystal structure of Polymerase I from phage G20c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-04 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 309.770, 98.011, 77.614 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 153.020 - 2.972 |
| R-factor | 0.2179 |
| Rwork | 0.216 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bwm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 153.020 | 3.254 |
| High resolution limit [Å] | 2.972 | 2.972 |
| Rmerge | 0.095 | 1.379 |
| Rmeas | 0.104 | 1.485 |
| Rpim | 0.039 | 0.548 |
| Number of reflections | 32681 | 1634 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 68.9 | 14.6 |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.3 M AmSO4 0.1 M Tris PH 8 0.05 M Li2SO4 0.02 M MgCl2 |
