7R0D
Structure of NUDT15 in complex with Geranyl monophosphate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.642, 48.269, 135.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 1.700 |
| Rwork | 0.184 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lpg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.715 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.640 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 34417 | 1759 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 11.3 | |
| CC(1/2) | 0.998 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris pH 8.5, 0.2 M sodium acetate, 30 % PEG4000 |
