7QZS

Crystal structure of mouse CNPase catalytic domain, G324D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	P 21 21 2
Unit cell lengths	68.630, 45.190, 61.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.740 - 1.800
R-factor	0.1997
Rwork	0.195
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xmi
RMSD bond length	0.011
RMSD bond angle	1.149
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.850
High resolution limit [Å]	1.800	1.800
Rmeas	0.150	2.927
Number of reflections	17959	1300
<I/σ(I)>	8	0.7
Completeness [%]	98.1	98.8
Redundancy	7.8	7.7
CC(1/2)	0.997	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	30% (v/v) Jeffamine ED-2003 and 0.1M HEPES (pH 7.0)