7QZP

Identification and characterization of an RRM-containing, ELAV-like, RNA binding protein in Acinetobacter Baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-02
Detector	Bruker PHOTON III
Wavelength(s)	1.5418
Spacegroup name	I 41
Unit cell lengths	69.560, 69.560, 32.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.870 - 1.650
R-factor	0.2131
Rwork	0.212
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fxl
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000	1.750
High resolution limit [Å]	1.650	1.650
Number of reflections	8220	857
<I/σ(I)>	18.76	1.7
Completeness [%]	87.1
Redundancy	11.7
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M sodium acetate trihydrate, 3 M sodium chloride