7QZJ
1.55 A X-ray crystallographic structure of SapH from Streptomyces sp. (HPH0547) involved in Pseudouridimycin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2020-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.581, 63.191, 211.415 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.470 - 1.550 |
| R-factor | 0.1606 |
| Rwork | 0.160 |
| R-free | 0.18230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ghg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.470 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.079 | 0.598 |
| Rpim | 0.046 | 0.509 |
| Number of reflections | 121033 | 5292 |
| <I/σ(I)> | 11.1 | 1.5 |
| Completeness [%] | 98.8 | 88.7 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.997 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M BTP pH 7.5 0.2M Na-K-Phosphate 27.5% PEG3350 |
