7QZD

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikF with an engineered HMA domain of Pikp-1 (Pikp-SNK-EKE) from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	67.381, 80.782, 103.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.830 - 2.200
R-factor	0.234
Rwork	0.231
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6w
RMSD bond length	0.009
RMSD bond angle	1.597
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	103.800	103.800	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.097	0.035	0.751
Rmeas	0.105	0.039	0.814
Rpim	0.038	0.015	0.302
Total number of observations	195704	2745	16870
Number of reflections	28553	457	2512
<I/σ(I)>	11.5	32.5	2.2
Completeness [%]	97.4	93.7	99
Redundancy	6.9	6	6.7
CC(1/2)	0.998	0.999	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NULL